Chemical genetic analysis of FTY720- and Ca 2+ -sensitive mutants reveals a functional connection between FTY720 and membrane trafficking

FTY720, a sphingosine-1-phosphate (S1P) analog, is used as an immune modulator for the treatment of multiple sclerosis. Collecting evidence has suggested mode of action of FTY720 independent of S1P modulators. In fission yeast, FTY720 induces increased levels of intracellular Ca2 + and ROS. We have previously identified 49 genes that lead to the elimination of FTY720 sensitivity.

Here, we characterized the mutant FTY720-sensitive in terms of its relevance to Ca2 + homeostasis and identified 16 FTY720- and Ca2 + – sensitive mutant (mutant FCS). Most of FTY720-sensitive mutants showed increased Ca2 + levels and exhibited Ca2 + dysregulation by FTY720 treatment. One functional category between a gene deletion makes the cells vulnerable to FTY720 and Ca2 + including membrane trafficking Golgi / endosomal.

Specifically, FTY720, but not phosphorylated FTY720 able to stimulate Ca2 + increase, inhibit secretion of acid phosphatase in wild type cells. Importantly, defective secretion of Golgi / endosomal trafficking mutants, Vps45, or Ryh1 deletion, which is further exacerbated by FTY720. Our FCS screen also identified a mutant protein adenylyl cyclase CAP1 related and homologous Rictor Ste20, the real elimination of aggravated decrease secretion of FTY720-sensitive. Collectively, our data may suggest the synergistic effects of FTY720 in combination with interference on proliferation and secretion of Ca2 + homeostasis.

Chemical genetic analysis of FTY720- and Ca 2+ -sensitive mutants reveals a functional connection between FTY720 and membrane trafficking
Chemical genetic analysis of FTY720- and Ca 2+ -sensitive mutants reveals a functional connection between FTY720 and membrane trafficking

Chemical and Microstructural Characterization of pH and [Ca 2+] Dependent Sol-Gel Transition in mucin Biopolymers

Mucus is responsible for controlling the transport and barrier function in biological systems, and its properties can be significantly influenced by changes in the composition and the environment. In this study, the effects of pH and CaCl2 checked on the transition-to-gel solution of mucin, the main structural component of mucus. micro structural changes that correlate with macroscale viscoelastic behavior as a function of pH and adding calcium to use rheology, dynamic light scattering, zeta potential, surface tension, and FTIR spectroscopic characterization. mucin solution switched from the solution to the behavior of gel between pH 4-5 and correspondingly shown an increase of more than tenfold viscoelastic modulus.

Addition of CaCl2 increase the sol-gel transition to a pH value of ca. 6, with a two-fold increase in the loss modulus at low frequencies and a tenfold increase in storage modulus. Changing special ionic conditions [H +] and [Ca2 +] -modulated sol-gel transition pH, isoelectric point, and the viscoelastic properties for such reversible conformational changes with mucin form a network structure via non-covalent cross-links between chains of mucin.

efficient strategies in enhancing sensitivity important for ultrasensitive detection of tumor markers. In this work, based on a strategy of chemical adsorption-assisted label conversion triggered electroactivity sensing interface, the Ag / AgCl were made to improve the sensitivity of built sandwich-type amperometric immunosensor for ultrasensitive detection of carbohydrate antigen 19-9 (CA19-9).

CD143 Blocking Peptide

20-abx062093
  • EUR 326.40
  • EUR 493.20
  • 1 mg
  • 5 mg

Anti-Hu CD143 Purified

11-796-C025 0.025 mg
EUR 118.8

Anti-Hu CD143 Purified

11-796-C100 0.1 mg
EUR 189.6

Anti-Hu CD143 PE

1P-796-T025 25 tests
EUR 168

Anti-Hu CD143 PE

1P-796-T100 100 tests
EUR 288

Angiotensin-Converting Enzyme (CD143) Antibody

20-abx007531
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Angiotensin-Converting Enzyme (CD143) Antibody

abx413653-1ml 1 ml
EUR 1228.8

Angiotensin-Converting Enzyme (CD143) Antibody (FITC)

abx413654-01mg 0.1 mg
EUR 693.6

Mouse Anti Human Cd143 Monoclonal Antibody

CABT-47050MH 1 ml
EUR 920.4

Mouse Anti Human Cd143 Monoclonal Antibody

CABT-47057MH 1 ml
EUR 920.4

Mouse Anti Human Cd143 Monoclonal Antibody,Biotin

CABT-47052MH 0.1 mg
EUR 889.2

Mouse Anti Human Cd143 Monoclonal Antibody,FITC

CABT-47054MH 0.1 mg
EUR 795.6

Mouse Anti Human Cd143 Monoclonal Antibody,RPE

CABT-47056MH 100 TEST
EUR 852

Purified Anti-human CD143 Antibody *5-369*

11430000 100 ug
EUR 365

APC Anti-human CD143 Antibody *5-369*

114301B0 25 tests
EUR 383

APC Anti-human CD143 Antibody *5-369*

114301B1 100 tests
EUR 995

APC Anti-human CD143 Antibody *5-369*

114301B2 500 tests
EUR 2984

FITC Anti-human CD143 Antibody *5-369*

114301G0 100 tests
EUR 547

FITC Anti-human CD143 Antibody *5-369*

114301G1 500 tests
EUR 2051

TRITC Anti-human CD143 Antibody *5-369*

114301H0 100 tests
EUR 547

TRITC Anti-human CD143 Antibody *5-369*

114301H1 500 tests
EUR 2051

PacBlue Anti-human CD143 Antibody *5-369*

114301I0 100 tests
EUR 547

PacBlue Anti-human CD143 Antibody *5-369*

114301I1 500 tests
EUR 2051

PacOrange Anti-human CD143 Antibody *5-369*

114301J0 100 tests
EUR 547

PacOrange Anti-human CD143 Antibody *5-369*

114301J1 500 tests
EUR 2051

PE Anti-human CD143 Antibody *5-369*

114301K0 25 tests
EUR 383

PE Anti-human CD143 Antibody *5-369*

114301K1 100 tests
EUR 995

PE Anti-human CD143 Antibody *5-369*

114301K2 500 tests
EUR 2984

PerCP Anti-human CD143 Antibody *5-369*

114301S0 25 tests
EUR 383

PerCP Anti-human CD143 Antibody *5-369*

114301S1 100 tests
EUR 995

PerCP Anti-human CD143 Antibody *5-369*

114301S2 500 tests
EUR 2984

APC/Cy7 Anti-human CD143 Antibody *5-369*

114301C0 25 tests
EUR 429

APC/Cy7 Anti-human CD143 Antibody *5-369*

114301C1 100 tests
EUR 1117

APC/Cy7 Anti-human CD143 Antibody *5-369*

114301C2 500 tests
EUR 3351

PE/Cy5 Anti-human CD143 Antibody *5-369*

114301L0 25 tests
EUR 429

PE/Cy5 Anti-human CD143 Antibody *5-369*

114301L1 100 tests
EUR 1117

PE/Cy5 Anti-human CD143 Antibody *5-369*

114301L2 500 tests
EUR 3351

PE/Cy7 Anti-human CD143 Antibody *5-369*

114301M0 25 tests
EUR 429

PE/Cy7 Anti-human CD143 Antibody *5-369*

114301M1 100 tests
EUR 1117

PE/Cy7 Anti-human CD143 Antibody *5-369*

114301M2 500 tests
EUR 3351

PerCP/Cy5.5 Anti-human CD143 Antibody *5-369*

114301T0 25 tests
EUR 429

PerCP/Cy5.5 Anti-human CD143 Antibody *5-369*

114301T1 100 tests
EUR 1117

PerCP/Cy5.5 Anti-human CD143 Antibody *5-369*

114301T2 500 tests
EUR 3351

iFluor® 350 Anti-human CD143 Antibody *5-369*

11430010 100 tests
EUR 547

iFluor® 350 Anti-human CD143 Antibody *5-369*

11430011 500 tests
EUR 2051

iFluor® 405 Anti-human CD143 Antibody *5-369*

11430020 100 tests
EUR 547

iFluor® 405 Anti-human CD143 Antibody *5-369*

11430021 500 tests
EUR 2051

iFluor® 430 Anti-human CD143 Antibody *5-369*

11430030 100 tests
EUR 547

iFluor® 430 Anti-human CD143 Antibody *5-369*

11430031 500 tests
EUR 2051

iFluor® 450 Anti-human CD143 Antibody *5-369*

11430040 100 tests
EUR 547

iFluor® 450 Anti-human CD143 Antibody *5-369*

11430041 500 tests
EUR 2051

iFluor® 488 Anti-human CD143 Antibody *5-369*

11430050 100 tests
EUR 547

iFluor® 488 Anti-human CD143 Antibody *5-369*

11430051 500 tests
EUR 2051

iFluor® 514 Anti-human CD143 Antibody *5-369*

11430060 100 tests
EUR 547

iFluor® 514 Anti-human CD143 Antibody *5-369*

11430061 500 tests
EUR 2051

iFluor® 532 Anti-human CD143 Antibody *5-369*

11430070 100 tests
EUR 547

iFluor® 532 Anti-human CD143 Antibody *5-369*

11430071 500 tests
EUR 2051

iFluor® 546 Anti-human CD143 Antibody *5-369*

11430080 100 tests
EUR 547

iFluor® 546 Anti-human CD143 Antibody *5-369*

11430081 500 tests
EUR 2051

iFluor® 555 Anti-human CD143 Antibody *5-369*

11430090 100 tests
EUR 547

iFluor® 555 Anti-human CD143 Antibody *5-369*

11430091 500 tests
EUR 2051

iFluor® 560 Anti-human CD143 Antibody *5-369*

114300A0 100 tests
EUR 547

iFluor® 560 Anti-human CD143 Antibody *5-369*

114300A1 500 tests
EUR 2051

iFluor® 568 Anti-human CD143 Antibody *5-369*

114300B0 100 tests
EUR 547

iFluor® 568 Anti-human CD143 Antibody *5-369*

114300B1 500 tests
EUR 2051

iFluor® 594 Anti-human CD143 Antibody *5-369*

114300C0 100 tests
EUR 547

iFluor® 594 Anti-human CD143 Antibody *5-369*

114300C1 500 tests
EUR 2051

iFluor® 610 Anti-human CD143 Antibody *5-369*

114300D0 100 tests
EUR 547

iFluor® 610 Anti-human CD143 Antibody *5-369*

114300D1 500 tests
EUR 2051

iFluor® 633 Anti-human CD143 Antibody *5-369*

114300E0 100 tests
EUR 547

iFluor® 633 Anti-human CD143 Antibody *5-369*

114300E1 500 tests
EUR 2051

iFluor® 647 Anti-human CD143 Antibody *5-369*

114300F0 100 tests
EUR 547

iFluor® 647 Anti-human CD143 Antibody *5-369*

114300F1 500 tests
EUR 2051

iFluor® 660 Anti-human CD143 Antibody *5-369*

114300G0 100 tests
EUR 547

iFluor® 660 Anti-human CD143 Antibody *5-369*

114300G1 500 tests
EUR 2051

iFluor® 670 Anti-human CD143 Antibody *5-369*

114300H0 100 tests
EUR 547

iFluor® 670 Anti-human CD143 Antibody *5-369*

114300H1 500 tests
EUR 2051

iFluor® 680 Anti-human CD143 Antibody *5-369*

114300I0 100 tests
EUR 547

iFluor® 680 Anti-human CD143 Antibody *5-369*

114300I1 500 tests
EUR 2051

iFluor® 700 Anti-human CD143 Antibody *5-369*

114300J0 100 tests
EUR 547

iFluor® 700 Anti-human CD143 Antibody *5-369*

114300J1 500 tests
EUR 2051

iFluor® 710 Anti-human CD143 Antibody *5-369*

114300K0 100 tests
EUR 547

iFluor® 710 Anti-human CD143 Antibody *5-369*

114300K1 500 tests
EUR 2051

iFluor® 750 Anti-human CD143 Antibody *5-369*

114300L0 100 tests
EUR 547

iFluor® 750 Anti-human CD143 Antibody *5-369*

114300L1 500 tests
EUR 2051

iFluor® 790 Anti-human CD143 Antibody *5-369*

114300M0 100 tests
EUR 547

iFluor® 790 Anti-human CD143 Antibody *5-369*

114300M1 500 tests
EUR 2051

iFluor® 800 Anti-human CD143 Antibody *5-369*

114300N0 100 tests
EUR 547

iFluor® 800 Anti-human CD143 Antibody *5-369*

114300N1 500 tests
EUR 2051

iFluor® 810 Anti-human CD143 Antibody *5-369*

114300O0 100 tests
EUR 547

iFluor® 810 Anti-human CD143 Antibody *5-369*

114300O1 500 tests
EUR 2051

iFluor® 820 Anti-human CD143 Antibody *5-369*

114300P0 100 tests
EUR 547

iFluor® 820 Anti-human CD143 Antibody *5-369*

114300P1 500 tests
EUR 2051

iFluor® 840 Anti-human CD143 Antibody *5-369*

114300Q0 100 tests
EUR 547

iFluor® 840 Anti-human CD143 Antibody *5-369*

114300Q1 500 tests
EUR 2051

iFluor® 860 Anti-human CD143 Antibody *5-369*

114300R0 100 tests
EUR 547

iFluor® 860 Anti-human CD143 Antibody *5-369*

114300R1 500 tests
EUR 2051

iFluor® A7 Anti-human CD143 Antibody *5-369*

114300S0 100 tests
EUR 547

iFluor® A7 Anti-human CD143 Antibody *5-369*

114300S1 500 tests
EUR 2051

mFluor™ UV375 Anti-human CD143 Antibody *5-369*

114300X0 100 tests
EUR 547

mFluor™ UV375 Anti-human CD143 Antibody *5-369*

114300X1 500 tests
EUR 2051

mFluor™ UV460 Anti-human CD143 Antibody *5-369*

114300Y0 100 tests
EUR 547

mFluor™ UV460 Anti-human CD143 Antibody *5-369*

114300Y1 500 tests
EUR 2051

mFluor™ Blue 570 Anti-human CD143 Antibody *5-369*

114300T0 100 tests
EUR 547

mFluor™ Blue 570 Anti-human CD143 Antibody *5-369*

114300T1 500 tests
EUR 2051

mFluor™ Green 620 Anti-human CD143 Antibody *5-369*

114300U0 100 tests
EUR 547

mFluor™ Green 620 Anti-human CD143 Antibody *5-369*

114300U1 500 tests
EUR 2051

mFluor™ Red 700 Anti-human CD143 Antibody *5-369*

114300V0 100 tests
EUR 547

mFluor™ Red 700 Anti-human CD143 Antibody *5-369*

114300V1 500 tests
EUR 2051

mFluor™ Red 780 Anti-human CD143 Antibody *5-369*

114300W0 100 tests
EUR 547

mFluor™ Red 780 Anti-human CD143 Antibody *5-369*

114300W1 500 tests
EUR 2051

mFluor™ Violet 450 Anti-human CD143 Antibody *5-369*

114300Z0 100 tests
EUR 547

mFluor™ Violet 450 Anti-human CD143 Antibody *5-369*

114300Z1 500 tests
EUR 2051

mFluor™ Violet 500 Anti-human CD143 Antibody *5-369*

11430100 100 tests
EUR 547

mFluor™ Violet 500 Anti-human CD143 Antibody *5-369*

11430101 500 tests
EUR 2051

mFluor™ Violet 510 Anti-human CD143 Antibody *5-369*

11430110 100 tests
EUR 547

mFluor™ Violet 510 Anti-human CD143 Antibody *5-369*

11430111 500 tests
EUR 2051

mFluor™ Violet 540 Anti-human CD143 Antibody *5-369*

11430120 100 tests
EUR 547

mFluor™ Violet 540 Anti-human CD143 Antibody *5-369*

11430121 500 tests
EUR 2051

APC/iFluor® 700 Anti-human CD143 Antibody *5-369*

114301E0 25 tests
EUR 429

APC/iFluor® 700 Anti-human CD143 Antibody *5-369*

114301E1 100 tests
EUR 1117

APC/iFluor® 700 Anti-human CD143 Antibody *5-369*

114301E2 500 tests
EUR 3351

APC/iFluor® 750 Anti-human CD143 Antibody *5-369*

114301F0 25 tests
EUR 429

APC/iFluor® 750 Anti-human CD143 Antibody *5-369*

114301F1 100 tests
EUR 1117

APC/iFluor® 750 Anti-human CD143 Antibody *5-369*

114301F2 500 tests
EUR 3351

PE/iFluor® 647 Anti-human CD143 Antibody *5-369*

114301P0 25 tests
EUR 429

PE/iFluor® 647 Anti-human CD143 Antibody *5-369*

114301P1 100 tests
EUR 1117

PE/iFluor® 647 Anti-human CD143 Antibody *5-369*

114301P2 500 tests
EUR 3351

PE/iFluor® 750 Anti-human CD143 Antibody *5-369*

114301Q0 25 tests
EUR 429

PE/iFluor® 750 Anti-human CD143 Antibody *5-369*

114301Q1 100 tests
EUR 1117

PE/iFluor® 750 Anti-human CD143 Antibody *5-369*

114301Q2 500 tests
EUR 3351

PE/Texas Red® Anti-human CD143 Antibody *5-369*

114301R0 25 tests
EUR 429

PE/Texas Red® Anti-human CD143 Antibody *5-369*

114301R1 100 tests
EUR 1117

PE/Texas Red® Anti-human CD143 Antibody *5-369*

114301R2 500 tests
EUR 3351

PE/iFluor® 700 Anti-human CD143 Antibody *5-369*

114301U0 25 tests
EUR 429

PE/iFluor® 700 Anti-human CD143 Antibody *5-369*

114301U1 100 tests
EUR 1117

PE/iFluor® 700 Anti-human CD143 Antibody *5-369*

114301U2 500 tests
EUR 3351

PE/iFluor® 594 Anti-human CD143 Antibody *5-369*

114301Y0 25 tests
EUR 429

PE/iFluor® 594 Anti-human CD143 Antibody *5-369*

114301Y1 100 tests
EUR 1117

PE/iFluor® 594 Anti-human CD143 Antibody *5-369*

114301Y2 500 tests
EUR 3351

XFD350 Anti-human CD143 Antibody *5-369, XFD350 Same Structure to Alexa Fluor™ 350*

11430130 100 tests
EUR 547

XFD350 Anti-human CD143 Antibody *5-369, XFD350 Same Structure to Alexa Fluor™ 350*

11430131 500 tests
EUR 2051

XFD488 Anti-human CD143 Antibody *5-369, XFD488 Same Structure to Alexa Fluor™ 488*

11430140 100 tests
EUR 547

XFD488 Anti-human CD143 Antibody *5-369, XFD488 Same Structure to Alexa Fluor™ 488*

11430141 500 tests
EUR 2051

XFD555 Anti-human CD143 Antibody *5-369, XFD555 Same Structure to Alexa Fluor™ 555*

11430150 100 tests
EUR 547

XFD555 Anti-human CD143 Antibody *5-369, XFD555 Same Structure to Alexa Fluor™ 555*

11430151 500 tests
EUR 2051

XFD594 Anti-human CD143 Antibody *5-369, XFD594 Same Structure to Alexa Fluor™ 594*

11430160 100 tests
EUR 547

XFD594 Anti-human CD143 Antibody *5-369, XFD594 Same Structure to Alexa Fluor™ 594*

11430161 500 tests
EUR 2051

XFD647 Anti-human CD143 Antibody *5-369, XFD647 Same Structure to Alexa Fluor™ 647*

11430170 100 tests
EUR 547

XFD647 Anti-human CD143 Antibody *5-369, XFD647 Same Structure to Alexa Fluor™ 647*

11430171 500 tests
EUR 2051

XFD680 Anti-human CD143 Antibody *5-369, XFD680 Same Structure to Alexa Fluor™ 680*

11430180 100 tests
EUR 547

XFD680 Anti-human CD143 Antibody *5-369, XFD680 Same Structure to Alexa Fluor™ 680*

11430181 500 tests
EUR 2051

XFD700 Anti-human CD143 Antibody *5-369, XFD700 Same Structure to Alexa Fluor™ 700*

11430190 100 tests
EUR 547

XFD700 Anti-human CD143 Antibody *5-369, XFD700 Same Structure to Alexa Fluor™ 700*

11430191 500 tests
EUR 2051

XFD750 Anti-human CD143 Antibody *5-369, XFD750 Same Structure to Alexa Fluor™ 750*

114301A0 100 tests
EUR 547

XFD750 Anti-human CD143 Antibody *5-369, XFD750 Same Structure to Alexa Fluor™ 750*

114301A1 500 tests
EUR 2051

APC/XFD750 Anti-human CD143 Antibody *5-369, XFD750 Same Structure to Alexa Fluor™ 750*

114301D0 25 tests
EUR 429

APC/XFD750 Anti-human CD143 Antibody *5-369, XFD750 Same Structure to Alexa Fluor™ 750*

114301D1 100 tests
EUR 1117

APC/XFD750 Anti-human CD143 Antibody *5-369, XFD750 Same Structure to Alexa Fluor™ 750*

114301D2 500 tests
EUR 3351

PE/XFD610 Anti-human CD143 Antibody *5-369, XFD610 Same Structure to Alexa Fluor™ 610*

114301N0 25 tests
EUR 429

PE/XFD610 Anti-human CD143 Antibody *5-369, XFD610 Same Structure to Alexa Fluor™ 610*

114301N1 100 tests
EUR 1117

Briefly, polydopamine-Ag nanoparticles (Ag NP PDA), a precursor signal, combined with labeled antibody served as a label and melamine graphene oxide (GO-MA) substrate with the absorption capacity of the chemicals applied as intelligent sensing interface. After a successful incubation label, there is no response primitive at this time because of poor conductivity between the label and the electrodes. However, the presence of H2O2, Ag NP of labels can be engraved Ag ions, which are absorbed by the GO-MA to establish GO-MA-Ag as electroactive substrates.